Journal: Materials Today Bio
Article Title: Carrier-free supramolecular architectonics of quercetin – berberine for safety-redefined therapy of ulcerative colitis
doi: 10.1016/j.mtbio.2026.102970
Figure Lengend Snippet: QB enhances epithelial barrier integrity and promotes colonic mucosal repair. A) Immunohistochemical staining of tight junction proteins Occludin and ZO-1 in colonic tissues (scale bar: 150 μm). B) Immunofluorescence staining of BrdU and β-catenin in colonic tissues (scale bar: 150 μm).
Article Snippet: Multiplex TSA-Based Immunofluorescence Staining: Dual immunofluorescence staining was performed on paraffin-embedded sections using primary antibodies against BrdU (Servicebio, GB12051, 1:200) and β-catenin (Proteintech, 51067-2-AP, 1:200), followed by HRP-conjugated secondary antibodies (Pinuofei Biotech) and TSA amplification with TYR-488 and TYR-555.
Techniques: Immunohistochemical staining, Staining, Immunofluorescence